Impact of monochromatic lights on the in vitro development of Cattleya walkeriana and effects on acclimatization

Abstract Light quality is an important factor for the adequacy of plant production through tissue culture, as it directly interferes with morphogenesis and photosynthetic capacity explants. The objective this study was to analyze impact monochromatic lights such light emitting diode (LED) on in vitro development Cattleya walkeriana G. their effects acclimatization. plants were developed under colors green, blue, yellow, red, 2 red:1 white LED lights. For cultivation, Knudson medium used, supplemented 20 mg L-1 sucrose, 5.5 agar, 2% activated charcoal, 100 mL coconut water, pH 6.0. ex acclimatized styrofoam trays containing sphagnum a substrate. In general, supplied lengths impactedin growth acclimatization analyses. There influence cuticle thickness vitro. Chlorophyll carotenoid contents not significant. We can conclude that formed by red yellow LEDs be indicated better performance C. walkeriana. blue provide superior than others, gains species provided possible hardening, which ensured greatest success seedlings during